Sakir Necat Yilmaz

3 Questions 2 Answers 0 Followers

Questions related from Sakir Necat Yilmaz

How can we demonstrate living cells in organotypic brain slice cultures?

Hi all We are planning to do organotypic brain slice cultures which will last about 6 weeks. How can we figure out whether cells in the slice are dead or alive? Thanks in advance.

18 September 2019 362 3 View

Can we perform intracellular staining of live cells for flow cytometry-cell sorting?

We're planning to sort live cells dissociated from mouse hippocampi according to nestin and GFAP reactivity. Does anybody have any experience with performing intracellular staining without...

28 April 2017 8,835 0 View

Have you ever performed debris elimination from dissociated brain tissues for flow cytometry-cell sorting?

We're trying to isolate neural stem/progenitor cells from subventricular zones and hippocampi as well as activated mature neurons from olfactorius bulbs of young mice. Debris (especially myelin)...

17 April 2016 5,291 2 View