hi everyone,
I have two questions-
1. Can we knock out the mouse gene in both cell- and tissue- specific manner?
2. If so, can we further get the mice whose CD4+ cells in spleen only are lack of the gene? Is this impossible due to the migration of wild-type CD4+ cells into spleen? Or aforementioned migration will never happen though the T cells will migrate out of secondary lymphoid organs once differentiated sufficiently.
Thanks, and any comments are welcome and appreciated.
Jiyuan