Jiyuan Liu

7 Questions 13 Answers 0 Followers

Questions related from Jiyuan Liu

How stable are CRE recombinase and TAM-inducible creER inside cells?

hi all, let's say mouse gene was mutated into promoter1-creER, and the creER following promoter1 was only expressed in newborn mouse, can I expected active CRE recombinase if I give the adault...

08 November 2019 8,348 0 View

Where would the compressed air be released in nebulizer?

Hi all, All paper I read emphasized on ventruli effect part of the nebulizer, But none addressed where would the compressed air go after it sucks up and atomizes the fluid containing drug of...

12 March 2019 3,408 0 View

Where do heart endothelial cells receive nutrients from? interstitial fluids or the blood flowing in artery?

hi all, I learned that heart endothelial cells are directly facing artery blood and supported by non-cell basement membrane. Thus it makes sense to me that it is more efficient for endothelial...

13 April 2018 833 1 View

Can in vivo passive diffusion permeability of cell plasma membrane be changed due to the change in cell membrane composition?

Hi all, it seems hard to find papers showing cell plasma membrane composition change induced by drug or biological process will alter the passive permeability in vivo. What I have got are-- 1....

19 February 2018 8,774 6 View

Could anti-CD28 help prevent activation induced cell death(AICD) while restimulation of Teff for measuring intracellular cytokine by flowcytometry?

Hi all, Please I need your expertise to solve a few questions as followings: 1. What should be the max percentage of dead cells acceptable when using flowcytometer to measure intracellular...

31 December 2017 6,592 4 View

Any literature validates the common RE-STIMULATION of effector T cells for intracellular cytokine determination?

Hi all, As a newbie, I am puzzled about two things though the re-stimulation is common for intracellular cytokine determination. 1. if re-stimulation will causeactivation-induced cell death...

28 December 2017 2,761 3 View

Can we knock out the mouse gene in both cell- and tissue- specific manner?

hi everyone, I have two questions- 1. Can we knock out the mouse gene in both cell- and tissue- specific manner? 2. If so, can we further get the mice whose CD4+ cells in spleen only are lack...

25 December 2017 7,603 3 View