I want to analyze total NTA (complexed with Fe + free NTA). Concentration may be upto 10 mM in water. Can anyone recommend the buffer, columns and conditions for HPLC analysis?
Hi Mr. Chandrashekar,
I was able to find a HPLC method for you, using Reverse Phase Ion Pairing... here is the link:
http://pubs.acs.org/doi/abs/10.1021/ac00236a053
Looks like any good C8 column will work well for you.
Paul
Thats good. I will look to standardize this method. Thanks Mr. Karch.
Hello Mr. Chandrashekar,
I want to analyze the complex Fe-NTA as well. The concentrations will be up to 0.1M in water. What column, detector and mobile phase did you use?
Thank you so much,
Fernando
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