Our ethyl acetate crude extract is not completely soluble in MeOH. I want to Know can we dissolve it in water and then fractionate it with MeOH/DCM system by using Silica gel column chromatography? what do you propose?
Thank you
You are using some dramatic changes in the polarity of your solvents so it might not be surprising if you end up with seemingly insoluble residues. However, depending on exactly what it is you are hoping to achieve this may not be a problem. It is difficult to be more specific as you provide no details as to what you are trying to do. What material are you extracting - plant? What class of compounds are you trying to extract and fractionate - lipid?
If it was extracted in ethyl acetate and pat is insoluble in MeOH then that part will not dissolve in water for the reasons given by Charles. And as he writes, that may or may not be a disadvantage depending on your purpose(s).
Check https://journals.plos.org/plosone/article/file?type=supplementary&id=info:doi/10.1371/journal.pone.0225056.s001
Also check https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3703421/
Hello Prof. Yaser,
Check on this
https://www.mdpi.com/2624-8549/3/3/62/htm
Charles H Hocart
Dear Prof. Charles,
My source is actinobacteria and I don't want to a specific class of compounds.
If you don't want a specific class of chemicals then what are you looking for? Are looking for a particular biological activity in your fractions?
Charles H Hocart
Yes, we want to evaluate their anticancer activity. We want to monitor the metabolite production ability of our strain and looking for new compounds, especially in ethyl acetate extract.
- Badges
- Science topic
- Similar topics
- Microbiology
- Assays