Please guide: Why my PCR lacks reproducibilty?

whenever i buy new primers (100pM) they work fine at first dilution (10pM) as well as first 3-4 polymerase chain reactions. But when i use same or new dilutions (10pM) after 15- 20 days at the...

20 July 2020 5,371 16 View

What can be possible reason when there is no reproducibilty in PCR which ones functioned?

When i run gradient PCR, it works well for 2-3 days, After that there is no amplification neither in same or other gradient ranges nor at temperature specified by first run. Is this issue with...

17 March 2020 1,643 2 View

What is the concept of mass collaboration ?

collaboration meaning

13 June 2019 4,233 3 View

What is the equivalent conditions for strongly \pi regular rings?

hello im looking for a equivalent conditions for strpngly \pi regular rings. a ring R is said to be strongly \pi regular if for each x in R there exist a y in R such that x^n = (x ^(n+1))y. do...

31 December 2018 9,118 3 View

Large eddy simulation in film cooling?

I am recently working on a large eddy simulation in film cooling process. I would like to ask you to guidance me in this field. Due to the high number of mesh and small time steps in large eddy...

25 November 2018 9,318 4 View

What are the considerations for Wind Tunnel Testing of a 152 mm chord length airfoil model in test section of 300mm x 100mm x 50mm test section?

I am working on an airfoil model of 152mm chord length x 46mm span. I have made a wind tunnel test section of 300mm x 100mm x 50mm.. It is a low subsonic WT upto 35 m/s. I want to compare the WT...

02 August 2018 4,322 3 View

Dose 'endomorphism ring of power series rings' has a special properties ?

hi i want to know about endomorphism ring of power series rings. if anyone know about that or road a paper about that please share it with me. thank you

28 April 2018 5,480 2 View

What are the analytical possibilities of a full-SEM (with LVs) method that are not available in EFA?

I have used a full SEM- (containing latent variables) for analysis. My Questions (See my simple example to better understand the questions.): Example: Consider three LV's A (with indicators a1,...

29 July 2017 832 5 View

I performing the test of moderation for Sex (Male- Female) in AMOS ?

29 January 2017 8,925 3 View

Do you know a good bioinformatic online site to predict the targets of chemical compounds?

I am looking for a reliable web site which I can give chemical name or molecular formula of compounds and software can identify the target proteins.

18 July 2016 3,297 6 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Does the circuit modeling for planar monopole antennas using Foster canonical forms give good approximation?

Hi researchers, I am searching for some accurate way to do the circuit modeling of planar CPW-fed monopole antennas. I have gone through the literature where mostly circuit modeling is done using...

28 February 2021 7,016 1 View