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Our ethyl acetate crude extract is not completely soluble in MeOH. I want to Know can we dissolve it in water and then fractionate it with MeOH/DCM system by using Silica gel column...
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Recently, to extract secondary metabolites from endophytic actinobacteria, I first frizzed-dry supernatant and then extracted it with ethyl acetate and methanol. But I did not have a good result...
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