Recently, to extract secondary metabolites from endophytic actinobacteria, I first frizzed-dry supernatant and then extracted it with ethyl acetate and methanol. But I did not have a good result for ethyl acetate. I am repeating the process and intend to use the liquid-liquid extraction method with ethyl acetate. The volume of my culture medium is 1 liter and I use a modified medium. What do you recommend?
Thank you.
Check https://www.sciencedirect.com/science/article/pii/S2590134620300025
Reflux extraction is more efficient than percolation or maceration and requires less extraction time and solvent. It cannot be used for the extraction of thermolabile natural products. https://cmjournal.biomedcentral.com/articles/10.1186/s13020-018-0177-x
Kindly check..
https://www.researchgate.net/deref/https%3A%2F%2Fwww.sciencedirect.com%2Fscience%2Farticle%2Fpii%2FS2590134620300025
Hello sir,
Kindly read this paper.
https://www.mdpi.com/2223-7747/9/9/1060/htm
Chinaza Godswill Awuchi Ca Dr. Gaurav Bhambri Kiprotich Kiptum
Thank you for your response. I want to extract metabolites from a bacteria that is grown in a culture medium. and I wanna know which one is better, liquid-liquid extraction of the aqueous phase with ethyl acetate or frizzed dry the aqueous phase and then extracts residue with ethyl acetate?
Do you know how much your secondary metabolite is produced in the producer? In addition to improve extraction efficiency, you can also increase the productivity by testing different fermentation media, temperatures, and time courses.
Rongfeng li No I don't. Now I just try to extract secondary metabolites from my bacteria and then evaluate their biological activity. Thank you, I will apply your comment.
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