Please guide: Why my PCR lacks reproducibilty?

whenever i buy new primers (100pM) they work fine at first dilution (10pM) as well as first 3-4 polymerase chain reactions. But when i use same or new dilutions (10pM) after 15- 20 days at the...

20 July 2020 5,371 16 View

What can be possible reason when there is no reproducibilty in PCR which ones functioned?

When i run gradient PCR, it works well for 2-3 days, After that there is no amplification neither in same or other gradient ranges nor at temperature specified by first run. Is this issue with...

17 March 2020 1,643 2 View

What is the concept of mass collaboration ?

collaboration meaning

13 June 2019 4,233 3 View

What is the equivalent conditions for strongly \pi regular rings?

hello im looking for a equivalent conditions for strpngly \pi regular rings. a ring R is said to be strongly \pi regular if for each x in R there exist a y in R such that x^n = (x ^(n+1))y. do...

31 December 2018 9,118 3 View

Large eddy simulation in film cooling?

I am recently working on a large eddy simulation in film cooling process. I would like to ask you to guidance me in this field. Due to the high number of mesh and small time steps in large eddy...

25 November 2018 9,318 4 View

What are the considerations for Wind Tunnel Testing of a 152 mm chord length airfoil model in test section of 300mm x 100mm x 50mm test section?

I am working on an airfoil model of 152mm chord length x 46mm span. I have made a wind tunnel test section of 300mm x 100mm x 50mm.. It is a low subsonic WT upto 35 m/s. I want to compare the WT...

02 August 2018 4,322 3 View

Dose 'endomorphism ring of power series rings' has a special properties ?

hi i want to know about endomorphism ring of power series rings. if anyone know about that or road a paper about that please share it with me. thank you

28 April 2018 5,480 2 View

What are the analytical possibilities of a full-SEM (with LVs) method that are not available in EFA?

I have used a full SEM- (containing latent variables) for analysis. My Questions (See my simple example to better understand the questions.): Example: Consider three LV's A (with indicators a1,...

29 July 2017 832 5 View

I performing the test of moderation for Sex (Male- Female) in AMOS ?

29 January 2017 8,925 3 View

Do you know a good bioinformatic online site to predict the targets of chemical compounds?

I am looking for a reliable web site which I can give chemical name or molecular formula of compounds and software can identify the target proteins.

18 July 2016 3,297 6 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Is the a way to contol flow (Flow velocity) without impacting pressure value?

I have a pipe with 10 mm in Diameter and 2 bar pressure, the fluid velocity is 200 m/s, the fluid is air. is there a way to reduce velocity without affecting the pressure value?

25 February 2021 7,187 3 View

Which software is best to analyse multi-phase fluid flows ?

Hello everyone, hope all is good. I want to analyse multi-phase fluid flow through pipe, which software is best to do so ?

21 February 2021 4,230 8 View

How to calculate the internal pressure of a riser pipe?

I am designing a riser pipe for use in ocean down to depths of 5km. Polymetallic nodules are pumped from the seabed to the surface. For my FEA and CFD analyses I need to input a maximum pressure...

06 February 2021 2,803 3 View

Is my beginners approach to ansys fluent udf implementation realistic?

Hello dear online comunity, i am new to CFD and we are currently involved in a project work for our masters degree. Since the problem we decided to simulate is quite complex, we cannot rely on the...

29 January 2021 2,200 3 View

Why can't ANSYS Mechanical APDL detect the volume of my design ?

The 1st image shows the moment when I import my design(IGES) into the Mechanical APDL. The 2nd image occurs when I wanted to Operate>Boolean>Divide Volume By Area. The design is a regular...

28 January 2021 2,962 3 View

I am trying to simulate phase change of refrigerant inside pipe extracting heat from panel in comsol. Can anyone help with how to simulate this?

My model is a rectangular multi layered structure under which refrigerant flows through cylindrical pipes. A heat flux of 600W/m2 applied on panel and internal forced convection is used for...

20 January 2021 9,478 1 View

Is it possible to entrain a particle(s) into a gap/crack/cavity within Ansys Fluent?

I am trying to simulate the entrainment of a discrete phase particle in Ansys Fluent. Essentially, the particles would enter a pipe containing water, and flow into a hole in the pipe, and become...

13 January 2021 4,789 3 View

Is there any pipeline for 16s metagenome bacterial classification based on their functions?

In 16s rDNA metagenome analysis, after sequencing data. I want to classify bacteria based on their functions. I am looking for a Pipeline or any software that can do this job?

13 January 2021 533 6 View

Is it possible to model a pressure-activated sealant in Ansys fluent?

I am trying to figure out how you'd model a pressure-activated sealant within Ansys fluent. This would be able to seal over a small crack, represented by a pressure differential, on the inside of...

12 January 2021 2,504 3 View