We submitted a sample of rice root for the purpose of quantifying phytohormones. Upon receiving the results, it was found that the sample contained BAP, a synthesized phytohormone that is typically supplied to plant tissue culture media. Can we detect and quantify 6BAP or BA?
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Raghad Mouhamad
- High-Performance Liquid Chromatography (HPLC):Widely used for phytohormone analysis. Separates and quantifies compounds based on their chemical properties. Requires a reference standard (pure 6BAP or BA) for accurate quantification.
- Mass Spectrometry (MS):Coupled with HPLC, MS provides high sensitivity and specificity. Identifies and quantifies compounds based on mass-to-charge ratios. Useful for detecting trace amounts.
- Enzyme-Linked Immunosorbent Assay (ELISA):Utilizes antibodies specific to 6BAP or BA. Provides qualitative or semi-quantitative results. Requires commercially available ELISA kits.
- Gas Chromatography-Mass Spectrometry (GC-MS):Suitable for volatile compounds. Requires derivatization of 6BAP or BA. Quantification based on mass spectra.
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