If a drug shows good anti inflammatory response in LPS induced inflammation in RAW 264.7 Cell lines and good hypoglycemic response in diabetic rats, does it work against diabetic neuropathy?
"Because hyperglycemia and inflammatory mediators are one of the major factors to develop diabetic neuropathic condition"
Dear Raghu,
RAW cell line could be just the beginning. Because it is immortal cell line that reflected the behavior of murine macrophages by a strong way. You have to use real regular rat cells especially DC and T cells for the applicable results.
Good luck.
I consider diabetic neuropathy as a late event in human - I am not sure how this is diagnosed or monitored in the rat model; I also do not know how good mechanisms of mouse immune cells, especially macrophages, can be extrapolated to human organisms (there have been findings 20 years ago on mouse NO pathways in macrophages, which have been under critical discussion, since a friend pointed out that human macrophages may not have the same pathway - but in principle, most pathways exist in humans and one can investigate and perhaps confirm them); on the other hand, I would consider anything which can help regulate blood glucose to normal levels, as "preventing" either diabetic neuropathy or preventing its progression. Not sure if you can achieve a cure of neuropathic conditions.
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