Expression study is one of the important tool to understand the pathways and network that might be involved in the disease biology. Initially Microarray tools were used then came REAL time PCR and now RNA Seq. All these tools inform about the genes that might have differential expression in disease like Rheumatoid Arthritis etc. How much variability can be expected if RNA seq is done in CEU population and lets say GIH population? Will the genes not be same in both population even if we consider the heterogeneous nature of disease like Rheumatoid Arthritis?
As far as my understanding goes, what you are expecting is differential expression - ya? If so, you might be comparing the expression of a gene from a tissue with its paired normal so that you can see the relative expression deregulation ( as common somatic event). So apparently, expression wise, its genetic make up should not effect much. But there comes the idea of eQTL, talks about variants that control the expression of that gene. So probably one might see, population specific signature of absolute expression change. These may not be somatic change, but probably confering population specific differential disease risk. So my belief is, population stratifican can have impact on overall expression profile.
Again, I think real time PCR (1984) is older technology than micro array(~1995) ("Invention of polymerase chain reaction (PCR) technology by Kary Mullis in 1984 gave birth to real-time PCR")
Thnx Mr. Sarkar and yes I didn't pay attention to the order in which these DE tools came, so thnx for the correction.
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