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Questions related from Sujit Kashyap
RNAseq was performed using Control and Knock down cells for a specific gene. Knock down was carried out using siRNA and samples were checked with Real time and semi quantitative PCR for knock down...
22 March 2017 735 4 View
I wish to study the effect of the monoclonal antibody against a certain protein. Can i purchase this monoclonal antibody for animal study from the widely known companies that are generally used...
03 November 2015 6,872 6 View
I want to check the effect of a drug that may affect some gene in brain by RT PCR. I can not have brain sample to see the expression changes but if that gene is expressed in blood then how...
19 January 2015 9,791 5 View
I have cultured RASF from synovial fluid of rheumatoid arthritis patients. Even after two passages I can see a lot of granules surrounding the fibroblast in the culture flask, I want to know if...
09 July 2014 9,592 3 View
Expression study is one of the important tool to understand the pathways and network that might be involved in the disease biology. Initially Microarray tools were used then came REAL time PCR and...
29 March 2014 5,772 2 View
Do we need a separate hood to avoid the mycoplasma contamination associated with the tissue culture?
29 October 2013 4,291 4 View
The role of environmental factors and smoking are supposed to be triggers but still we do not have a specific answer. It is reported that women are suffering with the disease three times compared...
07 June 2012 8,950 6 View
