Sujit Kashyap

6 Questions 10 Answers 0 Followers

Questions related from Sujit Kashyap

Why the percentage of reads aligned is so low?

RNAseq was performed using Control and Knock down cells for a specific gene. Knock down was carried out using siRNA and samples were checked with Real time and semi quantitative PCR for knock down...

22 March 2017 697 4 View

Can or should, a monoclonal antibody for western blot, IHC etc application be used for an animal study?

I wish to study the effect of the monoclonal antibody against a certain protein. Can i purchase this monoclonal antibody for animal study from the widely known companies that are generally used...

03 November 2015 6,844 6 View

How informative can WBC expression be to check the effect of a drug on a patient?

I want to check the effect of a drug that may affect some gene in brain by RT PCR. I can not have brain sample to see the expression changes but if that gene is expressed in blood then how...

19 January 2015 9,747 5 View

What might these dot like black granules in the rheumatoid arthritis synovial fibroblast culture be?

I have cultured RASF from synovial fluid of rheumatoid arthritis patients. Even after two passages I can see a lot of granules surrounding the fibroblast in the culture flask, I want to know if...

09 July 2014 9,559 3 View

Can we consider the RNA seq data produced in any part of the world to be true for any other population?

Expression study is one of the important tool to understand the pathways and network that might be involved in the disease biology. Initially Microarray tools were used then came REAL time PCR and...

29 March 2014 5,727 2 View

How to culture synovial fibroblast using synovial biopsy from rheumatoid arthritis patients?

Do we need a separate hood to avoid the mycoplasma contamination associated with the tissue culture?

29 October 2013 4,249 4 View