The problem is that skeletal muscle is non-dividing tissue, hence what is the meaning of telomere length?
One could actually argue that in non-dividing tissues, one could distinguish from "end-replication loss" of TL and oxidative strand breaks (and/or other processes that affect TL). If you would combine this with measures from other tissues (blood or skin) this is quite interesting, depends if you can biopsy and look at within-individual patterns, and/or what the underlying research interests are. Best of luck.
First of, the most common spelling is 'telomere'. Second, there are methods to determine length of telomeres and even company which is specializing exclusively in telomeres length measurement. Finally, telomeres are very important structural parts of chromosomes, they cap chromosomes protecting them from the ends, they involved in 3D organization of chromosomes in nucleus, they also have regulatory function through so-called heterochromatin position effect, and many other things.
We did measure human skeletal muscle telomere length by TRF assay. As far as I know we used standard published protocols for DNA extraction, digestion and Southern Blotting.
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