Which markers are candidates in mitochondria dysfunction in type 2 diabetic rat?

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Is Circadian rhythm important factor in night- active animal study such as rat?

The most studies which used animal model such as mice and rat, have been done their exercise training protocols during morning or noon however we know theses animals model are active at night. So,...

01 February 2018 7,702 9 View

Does anyone have any treadmill running protocols for type 2 diabetics mice?

Any reference for low and moderate endurance training and also HIT on motorized treadmill in type 2 diabetics mice? I couldn't use protocols based on Vo2. Thanks a lot in advance!

31 December 2015 5,264 5 View

Is it possible to using BSA or milk just for blocking and for primary and secondary antibody use only TBS-T?

Using TBS-T instead of BSA or milk.

04 May 2014 9,319 8 View

Can we assay telomere length in the skeletal muscle?

The problem is that skeletal muscle is non-dividing tissue, hence what is the meaning of telomere length?

04 May 2014 4,396 4 View

How to conduct a mitochondrial dysfunction study by PCR?

I know there are a number of protocols and techniques for studying mitochondrial dysfunction and apoptosis such as TUNEL assays, immunoblots and so on. Can we use PCR for this purpose? What...

04 May 2014 4,026 2 View

Tissue prepration fpr ELISA Test?

Happy new year! Do we have to consider different tissue preparation for ELISA in skeletal muscle, liver and heart of mice? Same question about analyzing different protein and enzyme (PGC-1a,...

01 January 1970 4,495 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

How do I collect online measures in a same-different task?

The same-different task requires to subjects to indicate whether a pair of stimuli seen or heard are the same (say AA or BB) or different (say AB or BA). Researchers often collect offline measures...

20 February 2021 9,226 2 View

Do I need to introduce HFD to ob/ob mice to obtain a T2DM model?

I am planning to work on T2 diabetic animals and check the efficacy of probiotics. My initial plan is to fed B6 animals with HFD for a while. But now, I have the chance to work on ob/ob mice. My...

18 February 2021 6,896 3 View

Can I get the design of circular cylinder to fix thermocouple on the surface and cartridge heater in core ?

Respected Scientific Community. I'm interested to carry out experimental studies on a circular cylinder. But even after doing literature review I unable to get the exact design of the cylinder...

10 February 2021 6,221 1 View

What are the applicable reinforcement learning based control methods for systems that have control delay?

I am studying the experimental application of reinforcement learning (RL) based control methods. For that reason, I modeled a nonlinear system without delay and trained the RL agent. In this...

02 February 2021 7,999 3 View

Can I heat my ELISA at 37C?

Hello, I am conducting a sandwich-type ELISA. It is to see if small molecules can interfere with a protein-protein interaction. As this interaction would occur in the human body, may I heat...

31 January 2021 4,271 3 View

How to get the insoluble fraction of PK digestion for mitochondria?

I am trying to check for some proteins if they are located in the inner or outer mitochondria membrane after treating the cells with a neurotoxin. I am using PK to digest the mitochondria, but I...

27 January 2021 5,145 2 View

How seniors face covid 19 without fear?

Seniors in the world with diabetics and hypertension and heart diseases are millions ,and they are facing covid.therefore we should give them advices and precausions to face covid 19,this...

26 January 2021 8,349 4 View

The litter effect and what can I do to avoid it?

My thesis involves experimental study with Wistar puppies. I am dividing the puppies equally in relation to the groups. However I only have 4 mothers and I will use all of their puppies. There is...

25 January 2021 6,965 3 View