Can we add tris buffer powder while preparing dehydrated culture media as buffering agent? or we have to prepare the solution only?
Is it for animal cell culture? The question is not clear.
Tris is not a preferred buffer for animal cell culture media. Sodium bicarbonate/ CO2 system or Hepes are generally used buffering systems.
You could use HEPES with humidifier if you don't want to use the CO2 incubator
one can use HEPES buffer and Sodium Bicorbonate for buffereing . Another alternate could be Phosphate buffer saline (PBS).
Thank you for the info. But i want to work with TRIS buffer system only, thus need to know if anyone has tried using it before
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