How to Accelerate / increase growth initiation of aerobic bacteria on solid media? Known bacteria & known media - yet the growth is slow than recommended, what else can be added to start up the bacterial growth on the media, growth factors or anything else? if so which growth factors?
Start by varying your incubation temperature, not all organisms benefit from growth at 37 degrees C (4, 23, 28, 37, and 42 degrees C are incubation temps. that I find helpful). Salinity content may also be of importance depending on the organisms you wish to culture. If they are aerobic, and not fastidious bacteria, they should be readily cultured on TSB with blood once an ideal incubation temperature is determined.
If that does not work, the organism is likely fastidious and could require factors NAD and hemin. Chocolate Agar is probably the best place to start. Other additives to consider are Tween 80 and Vitamin K, which are easy to work with but cannot be added to already prepared media. If you get that far you will likely need to start working with controlled aerobic/anaerobic environments with specific CO2 content.
pH of media is correct. temperature for incubation is well maintained yet growth is slow.
can we add anything to accelerate its growth?
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