04 June 2019 1 4K Report

We are spiking human serum with synthetic 25-hydroxyvitamin D and wanted to determine whether the vitamin D binding protein would be able to bind this exogenous synthetic 25-hydroxyvitamin D...

Any ideas on whether that happens and possibly how to validate it?

More Salma Tammam's questions See All
How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

How long does it take to design and validate transition state analogue inhibitors?

Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...

14 February 2021 2,610 1 View

Hyperspectral Image : open, visualize, process in Python?

I am looking for a tutorial or course to learn how to open, visualize , process hyperspectral images in .hdr , .raw format using python. Any recommendation for a tutorial is appreciated ? Thank...

08 February 2021 7,896 3 View

Frequency calculation with TDDFT???

Hi, I have been trying to calculate the frequency vibrations of the excited state S1 of an organic molecule (24 atoms). The geometry optimisation of the excited state converged smoothly howerver...

22 January 2021 1,463 3 View

Should I do a quality assessment for studies that will be included in a systematic review, even if I am not doing meta-analysis?

The types of studies included are case reports, case series, epidemiological studies. The information collected will probably be analyzed qualitatively only.

17 August 2020 3,628 7 View

How to interpret DSC heat flow curves?

I'm running DSC for a mixture of 2 polymers and I need help to interpret the heat flow curves

13 August 2020 2,993 4 View

Pescadf output interpretation?

i am working on panel data having cross sectional dependence problem . as it shows cross sectional dependence by xtcdf command ,presence of cross sectional dependence demands pescadf and cips unit...

03 August 2020 6,072 1 View

Does anyone have a MATLAB code of PV module? (Plotting the module's output)?

Hello, I am trying to model a PV module in MATLAB that can get 24h data inputs of (irradiance, air temperature and wind speed) to produce the output.

02 July 2020 5,890 2 View

Problem with MG and PMG model in stata?

i am using panel data with three groups A=15 B =37 C =12 cross section data 1990-2019 running the following codes in stata 14 xtpmg d.lip d.lop d.lex, ec(ec) lr(l.lip lop lex ) mg replace xtpmg...

02 July 2020 5,777 3 View

Covid-19 Are serological test relevant to mass screening?

What is the time required for the body to produce a detectable concentration of anti SARS-CoV-2 ? Would such lag time make serological tests less interesting from a screening perspective? Should...

27 March 2020 9,350 3 View

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Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

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