Can I use p-phenylenediamine as a antifade for live cell immunofluorescence or it presents some cytotoxic effect? If yes, which is the ideal concentration?
Honestly:
I think (hopefully I am correct with this) it is possible. I suggest reading some literature and ads /product data sheets of companies dealing with the issue: instead of mentioning them: Please, just search "Google" or an other Data base / Search Machine for phrase:
| PPD antifade reagent additive live fluoresc* micr* | (or variants) and find a lot of sources.... (also some ResearchGate threads ).
As always, there are at least some solutions for your problem and therefore other anti-Fading reagents too... but only to name one or two in this moment:
cf:
Krenik et aI., 1989 (: ‚Comparison of antifading agents used in immunofluorescence‘ in: J. Immunol. Methods 117(1) 91-97)
Cf.: (prior to accessing in your browser, delete: ‚___‘ in between >> https: and //pubmed….
Be aware that in my experience in developing antifade mountants (at Molecular Probes / Thermo Fisher Scientific) I have found that PPD oxidizes quickly and becomes brown in solution. Not a great thing. So if you use it, you'll want to use it right away. But I have no experience trying it for live cell systems. I suspect it would be toxic.
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