Hi there to all of you.
Can I extract proteins with TRIZol? If so, what would be the most effective way to measure proteins following TRIZol extraction? I intend to detect proteins using western blot after using the TRIzol reagent from Ambion.
The issue is that, in order to avoid having to redo the experiment, I now need to obtain the mRNA for qRT-PCR and the protein for Western blot from the same sample. Viral proteins within infected cell lines are the proteins I'm looking for.
Hi,
You can extract total protein from the lower phase (organic phase). During RNA extraction, separate the aqueous phase for RNA extraction and collect the red phase (Organic/Lower phase) for protein extraction following the Trizol protocol. Simply follow the manufacturer's instructions, which can be found here: https://assets.thermofisher.com/TFS-Assets/LSG/manuals/trizol_reagent.pdf
Best regards!
Did it work? I've heard that protein extraction with TRIzol is not as efficient as it sounds and it is not the best for western blot, so I'm interested in knowing if it is true
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