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Questions related from Alaa Al-Emerieen
Hi there to all of you. Can I extract proteins with TRIZol? If so, what would be the most effective way to measure proteins following TRIZol extraction? I intend to detect proteins using western...
24 November 2023 1,184 3 View
Hello Everyone, I am trying to extract viral RNA from infected Thp1 cell cultures. I thought I could use TriZole for cell homogenization. can I combine two methods: trizole to keep the samples...
02 October 2023 3,059 3 View
Hello , I am having trouble in differentiating U937 cells. I tried using various incubation periods and different PMA dosages—10, 20, and 100 ng—but it seems like many of the cells are dying...
11 July 2023 2,343 4 View
I'm trying to revive U937 cells, but I'm having a problem getting the cell line to develop normally. The growth media is RPMI-1640 from Sigma Aldrich with L-glutamine, 10% FBS added, as well as...
06 February 2023 1,542 2 View
I started working in a U937 line. On the day I thawed the cryopreserved vial of cells they were nicely viable. When I checked them the next day, they were at 30 % viability. and shriveled. Has...
11 January 2023 1,661 2 View