Alaa Al-Emerieen

3 Questions 4 Answers 0 Followers

Questions related from Alaa Al-Emerieen

Any advices for differentiating U937 into M0 macrophages?

Hello , I am having trouble in differentiating U937 cells. I tried using various incubation periods and different PMA dosages—10, 20, and 100 ng—but it seems like many of the cells are dying...

11 July 2023 2,282 4 View

What could be the problem in reviving U937 ?

I'm trying to revive U937 cells, but I'm having a problem getting the cell line to develop normally. The growth media is RPMI-1640 from Sigma Aldrich with L-glutamine, 10% FBS added, as well as...

06 February 2023 1,496 2 View

Does the viability of U937 cells drop notably after cryopreservation?

I started working in a U937 line. On the day I thawed the cryopreserved vial of cells they were nicely viable. When I checked them the next day, they were at 30 % viability. and shriveled. Has...

11 January 2023 1,656 2 View