It might be a silly question, but for many biochemical assays like ROS analysis, the results are generally normalised to the no. of viable cells in the cell culture samples of interest, keeping also in view the toxic effect of the experimental treatments the experimentor is studying the response towards. Alternatively, some protocols choose normalising these results to the total protein concentration of the sample instead of teh no. of viable cells. I wondered if both, the no of viable cells and total protein levels of the sample be relatable thus? My doubt concerns the tremendous potential of the cellular proteins to fluctuate (upregulate or downregulate) in response to a treatment, in comaprison to the normal controls, thus may be not making protein concentrations a suitable candidate for the normalisation.
Thanks.
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