Poojashri Mishra

4 Questions 22 Answers 0 Followers

Questions related from Poojashri Mishra

Can the total protein pool from a sample loosely indicate the no. of viable cells?

It might be a silly question, but for many biochemical assays like ROS analysis, the results are generally normalised to the no. of viable cells in the cell culture samples of interest, keeping...

07 July 2016 5,247 1 View

Can anyone suggest why I do not see amplification in Real Time PCR?

I am trying to standardise my Tqman based qRT-PCR for TNF-alpha in Astrocyte cultures, but unable to do so. The starting primer and probe concentrations were 700 nM, tapered down to 300nM, to no...

01 January 2015 6,762 9 View

How does one deal with the fibroblast contamination in glial cultures?

I culture glial cells from the spinal cords of neonatal wistar rat pups. The problem is, however I try to remove the meninges from the spinal cord, I find it tricky to be able to completely get...

12 December 2013 3,491 6 View

Troubleshooting regarding obtaining a pure microglial culture by mild trypsinization?

I am working towards establishing pure microglial cultures using the mild trypsinization protocol described by Saura et al., 2003 and successfully used by many labs. However, I am facing issues in...

09 September 2013 1,778 23 View