I've performed Bradford assays in parallel with other cell viability assays. Can the level of protein in cell lysates be used as an indicator of cell viability?
No. One cannot. This is because the level of protein in the lysate vary dramatically with the isolation method, batch variation, environmental condition etc. Furthermore, it is not sensitive enough. Lets say, if you compare the protein content of a live cell, an apoptotic cell and degenerating cell....you can expect cell lysate to give an accurate method.
No. One cannot. This is because the level of protein in the lysate vary dramatically with the isolation method, batch variation, environmental condition etc. Furthermore, it is not sensitive enough. Lets say, if you compare the protein content of a live cell, an apoptotic cell and degenerating cell....you can expect cell lysate to give an accurate method.
I agree with Mukesh, you can't use Bradford porotein quatntification for the evaluation of the cell death (at least for a small time). One easy approach ius the use of propidium iodide in flow cyotmoetry for example or the use of specific viability test such as XTT.
have a nice day
Olivier
Theoretically, yes, but it looks time- and work-consuming. If you do some toxicity assay, there are a lot of possibilities. What is your aim? What kind of samples would you like to measure?
There is a fast and direct method for protein quantification based on Fourier transform infrared spectroscopy (FTIR). It's less sensitive than Bradford (protein concentration should be higher than 250 μg / mL) but You don't need calibration and it's independent of detergents. In your case, i think you can manage to have concentrated sample. So, it worths to try.
Use the MTT test, is cheap and fast. Always we use a death control (saponin) as blank
MTT assay is the reliable and fast method to measure the cell viability. You can go for it
SRB (Sulphorhodamine B) method measure directly the plate bound protein content of an experimental well. It corresponds to the attaching living cells. It is a well-known toxicity assay.
- Badges
- Science topic