Sililated organic extract GC-MS, Kovats index or Van den Dool and Kratz index ??

dear colleagues, I'm doing a GC-MS analysis of presilylated extracts with BSTFA (essentially polyphenols) and i want to determinate an Index to compare with adams book (2007), which index should I...

09 October 2019 4,144 2 View

How to Interpret ANOVA's results ?

Dear colleagues I have made two ways ANOVAs and post hoc tests, I have obtained the ANOVA's table (SCE, f & p values) with approximatively similar values but the entered responses were different,...

09 October 2019 2,204 8 View

Which reagent is better for lipids HPTLC revelation ?

Dear colleagues, I'm doing an HPTLC of several plant oils to compare their lipidic profiles, i have two protocoles the first one with sulfuric-vanillic acid, the second with phosphomolibdic acid....

08 September 2019 4,748 5 View

ABTS radical scavenging protocole modification ?

dear collegues, i would like to study the radical scavenging effect of some plant extracts against ABTS, and i would like to know if i can change the sodium persulfate with sodium sulfate and the...

06 July 2019 3,518 4 View

How can I interpret the GC-MS results obtained after sililation of an extract???

Dear collegues. I have analysed some extracts by GC-MS after sililation and i have obtained my report after comparing the results with the NIST database, Before doing more bibliographic...

02 March 2019 4,192 4 View

ABTS or DPPH: Which antioxydant assay is the best for an bioautography test on TLC?

Dear collegues, I'm doing an HP-TLC of some extracts and would like to do an antioxydant screening on plate (DPPH-TLC or ABTS-TLC) I would like to know wich one is the best and why ??

31 December 2018 9,829 4 View

Comparison between the Hydrodistillation & SFE extracted essential oils

dear colleagues. I would like to know what is the difference between SFE and hydrodistillation extracted essential oils, i have heard that SFE EO cannot be defined as essential oils according to...

01 January 1970 2,544 3 View

Payed publications VS open acess ? do you think we will still have payed publications after 20 years ?

After the use of paper hack tools such as sci-hub the majority of journals prefer the open-access format where the authors pay the publication to be shared. Do you think we ll still have pay to...

01 January 1970 9,435 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...

24 February 2021 2,602 1 View

How to prepare a thin polymer film by post-polymerization/crosslinking?

24 February 2021 2,362 5 View

Is is possible to pole with corona poling pellets of the sample material instead of thin films?

As it is highly probable that pellets will have a higher degree of air gaps and voids than thin films, is it possible to pole the samples with corona poling nevertheless? Or is there the...

24 February 2021 1,349 1 View

Any easy method of analyzing vitamin D in foods by HPLC?

Looking for method of extracting and analyzing vitamin D in foods by HPLC

24 February 2021 7,524 7 View

What HPLC Marker(s) should be used to detect anthocyanins in a sample?

What HPLC Marker(s) or standard is used to detect and quantify anthocyanins such as Delphinidin, Cyanidin in a given sample of plant extract?

23 February 2021 1,832 4 View