i'm doing TLCs and visualising my plates under 254nm & 366nm, I would like to know if it's possible to correlate the fluoresent colorations i obtain with families of secondary metabolites.
no, can not
this impossible, you most depend of other characters like Rf. and you most present standard compound if the compound that you need to detect by color, didn't know their color previously,
thatks
Thank you Mr. Kadhum, i saw that chlorophylles are red in 366nm and some other compounds have specific colors in this lenghtwave, that's why i'm asking.
Check their fluorescence spectra and make some review first from previous literature.
Dear mr. Grzegorz Boczkaj & Mohd AZRIE Awang ,
I had made some bibliographic researches but i find that somes says that there is specification others says that there is no, that made me a little bit confused. i'm using 366nm UV lamp on a normal phase aluminium silica plate. is there a book you recommand me that gives some indications about metabolite's fluorescence under 366nm.
Best regards
Dear Micham,
I have done a brief literature search and I can reassure you. The tagline ‘Fluorescence spectroscopy’ reveals a large set of relevant papers on both Google and Google Scholar.
Best Regards
David.
- Badges
- Science topic