I need to measure RFP (red fluorescent protein) induction in FFPE sections. I know RFP is expressed by fluorescence when I dissect the brain, I just need to make some pretty-looking slides.
While computing the mechanism of a trimethylsilylcyanation related to this work (10.1021/acs.organomet.1c00070) I managed to optimize the geometry of both reactants and product and locate a good...
26 May 2024 9,925 0 View
Hi, I want to isolate nuclear proteins from the diatom P. tricornutum. I can successfully isolate the nuclei, and can image them under the microscope. But I fail to lyse the nuclei. As they were...
14 December 2023 3,640 1 View
I am looking for high molecular weight polystyrene for technical applications. The molecular weight should be at least 500.000 g/mol or higher. The PDI is not that important. Can anyone give me...
29 August 2023 7,516 2 View
Dear all, Our group has tried for several months to establish a homemade protocol for MDA-assay (Malondialdehyde Colorimetric Assay) to measure lipid peroxidation levels in cell culture. So far,...
29 August 2023 7,697 3 View
Dear all, I am curious to learn about the identification of the pictured salp individuum. The pictures were taken in October 2022 near San Antonio del Sur (southern Cuba) not far away from shore....
15 August 2023 9,008 0 View
According to a paper published in 50th IWCS conference "Sag-Tension Calculations for Aerial Cable with Linear or Nonlinear Tensile Properties" by T. C. Chu, there are simple equations with one...
06 August 2023 5,991 0 View
I received a text request from Kim Lawson about ketamine in fibromyalgia. I do not find how to send as I do not have Twitter, nor Kim's email
18 July 2023 4,146 1 View
hi community, I would like simulate the pump down graph of my vacuum pump. My first test is to use laminar flow, create an air cube of 1m^3 and add a vacuum pump on a side. The specificity of the...
13 June 2023 8,974 1 View
Hi, I would like to use mEOS to tag proteins to evaluate their subcellular localization. I am new to PALM, and am a bit confused with all the different versions of mEOS available... mEOS2? mEOS4?...
19 April 2023 7,456 1 View
Dear colleagues, On an expedition in the southern Sargasso Sea, subtropical Northwest Atlantic, I encountered repeatedly specimens of the photographed heteropod, never (so far) being bigger than...
28 March 2023 3,057 4 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I am doing immunohistochemistry of the c-Fos protein and I have a budget problem. For context, the experiment has been done over 3 years prior to my arrival and I have 40 brains done and 100 left....
29 July 2024 5,961 2 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I designed 2 dual hybridization probes for my single SNP. where I attach my fluorophore 5'-FAM-nnnn-BHQ1-3', and 5'-HEX-nnnnnn-BHQ2-3'. I need to run it on the MIC RT PCR machine by Biomolecular...
28 July 2024 5,658 0 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...
27 July 2024 6,605 0 View
It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...
25 July 2024 8,738 0 View