I am looking for a non-NMR based (preferably) method of analyzing glycogen synthesis in the skeletal muscle and heart. Can anyone share a protocol where one has used radiolabeled glucose incorporation into muscle glycogen in vivo?
I know nothing about radiolabeled glucose incorporation, though I did read an interesting approach using ultrasound to assess the level of glycogen in skeletal muscles a few years back. I don't remember the author off the top of my head but I suspect it would be easy to find. The authors validated their algorithms using muscle biopses.
Dan Robbins Thank you for your reply. Yes, I am aware of that paper that you mentioned. But I am really looking for more of a "real-time" method of glycogen synthesis.