Can someone recommend optimized protocols on amlyoid monomerization, solvents for making solutions, gel running conditions and maybe some mass specific identification?

More Gerry A Quinn's questions See All

Can anyone tell me the largest number of proteins directly extracted from soil ?

Can anyone tell me the largest number of proteins directly extracted from soil ? I am writing a paper about soil protein extraction and wanted to know what sort of yields other researchers were...

31 December 2019 8,094 2 View

We are looking for a Candicidin D standard ?

We are looking for a Candicidin D standard for HPLC and mass spectrometry. It is no longer made by the company listed on PubChem (alpha chemistry). Does anybody have any other suggestions?

31 December 2018 4,426 3 View

Are there specialised methods for extracting DNA from alkaline soils ?

I am conducting a metagenomic analysis of alkaline soil and I wondered if there were any specialised methods of DNA extraction given the alkaline nature of the soil.

07 August 2018 3,257 4 View

What is the maximum growth pH recorded for Streptomyces on solid media ?

I have been characterising a new strain of Streptomyces and was wanting to compare it to other similar alkaliphilic strains of Streptomyces. I have read of figures as high as 11.5 but the...

06 July 2018 1,556 8 View

Can you report a new bacteria on whole genome sequencing alone or do you have to have some polyphasic studies ?

I am being asked to provide polyphasic studies to name a new bacterial species, however, I have already provided the whole genomic sequence together with some comparitive genome analysis and some...

06 July 2018 2,071 0 View

What is the maximum pH level tolerated by Streptomyces coelicolor M145 ?

Are there any references to the maximum pH levels tolerated by strain S. coelicolor M145 ?

03 April 2018 2,601 4 View

What is the average difference in pH of agar before and after sterilisation ?

I had previously made a series of (modified SFM) agars at high alkaline pH 8----->13.6. I did not measure the pH of the resulting solidified agar because it just seemed a bit awkward and I...

02 March 2018 862 4 View

I was wondering if pH regulation in the region of amyloid deposition influences the development of Alzheimers ?

I was wondering if the pH of an environment influences the development of amyloid proteins ? Cellular control of pH is performed by porters/ antiporters. I have searched for these in human...

10 November 2017 9,375 4 View

What is the purpose of multiple copies of antibiotic resistance genes ?

We have sequenced a bacteria and found multiple copies of antibiotic resistance genes. The sequences of the copies are not identical. I was wondering if this diversity of copies is to increase the...

09 October 2017 6,473 13 View

How can you recover a protein sample which is tightly bound to a graphite column ?

I have been using activated carbon columns to clean up protein samples. Protein recovery has been very low. When I use a large amount of protein i.e. 5mg the recovery is only 0.5mg . I was...

09 October 2017 501 1 View

Can I proceed with Response Surface Methodology (RSM) with only one factor for further optimization?

I went for Plackett Burman Design for selecting the optimization factors of growth conditions for pesticide degradation. I started with 7 parameters. But, after the experiments and analysis, only...

29 July 2024 5,015 1 View

I did hirshfeld surface analysis for molecule but did not find red spot and interaction surface in energy frame work, is it ok for research article ?

In hirshfeld surface calculation i did not find any red spot in hirshfeld surface, is it ok for research article to write?

28 July 2024 2,988 0 View

Why specific capacitance is not proportional with the specific surface area?

Dear researchers, Why specific capacitance is not proportional to the specific surface area? I have material A with a surface area higher than material B, but material B is showing little bit...

26 July 2024 9,156 2 View

Differences between Surface Tension and Interfacial Tension in Liquid-Gas Phases?

Hi everyone, I have a question regarding surface and interfacial tension. Although I understand that these tensions are studied for different purposes, can they be considered equivalent in the...

18 July 2024 382 5 View

Comparison of tissue to serum MS data?

Hello, So I have mouse brain tissue MS data from one of my predecessors and I have human serum data from the hospital that I collaborate with. We are trying to find markers for the diagnosis of...

11 July 2024 8,484 0 View

Roughening Silver Electrode Using CH Instruments for SERS applications?

I am in the process of roughening a silver electrode for SERS measurements using the latest version of CH Instruments. Most procedures in the literature mention sweeping the potential from -0.3 V...

30 June 2024 4,639 3 View

How closely does an elastic sheet conform to what's below it?

See the attached file for an illustration of this problem. Consider an elastic sheet S (orange) that’s laying atop a solid base B (blue). The sheet is under tension (arrows) and is held to the...

30 June 2024 747 4 View

My question is about a barley seed ?

I need some articles on the analysis of the nutritional quality of barley (H.vulgare.L) and the analytical approach of metabolomics and proteomics.

26 June 2024 3,451 2 View

How can we inhibit the activity of acetylcholinesterase to alleviate the symptoms of Alzheimer pathology?

With a stressful lifestyle, we start experiencing forgetfulness at an early age, which can accelerate the onset of Alzheimer's disease symptoms in young people as early as their 30 years old....

24 June 2024 938 1 View

What kind of surface morphology must be there for good photocatalytic activity of thin film?

If I want to increase the photocatalytic activity of a thin film sample what should be it's ideal surface morphology and parameters? All scientific answers related this are highly appreciated.

19 June 2024 6,744 0 View