Can someone recommend a DNA sequence for a glycine/serine linker for optimal fusion protein expression in HEK293t cells?

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Can any one recommend a company that produces proteins of good purity for a reasonable price?

I have some recombinant DNA that I would like to have expressed in fairly large amounts (~50mg). The protein is for a putative vaccine.

07 August 2017 6,648 5 View

Does anyone have a protocol for formalin-killed Bordetella pertussis cells

I have a source of live pertussis cells thanks to ReseachGate. I'm now looking for a protocol to kill them using formalin.

02 March 2017 2,418 4 View

Does anyone have any experience using Click-iT EdU imaging kits for lymphocyte proliferation assays, if so what cell culture media do you recommend?

What cell densities do you recommend.

01 February 2017 4,310 4 View

Does anyone know where I can get heat-killed or formalin-killed Bordetella pertussis cells?

I want to use the killed bacteria as an additional adjuvant in a vaccine trial in rats.

01 February 2017 5,560 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Chris Cromwell

Hi Leanne Dooley

There are a variety of codon optimization tools which can help you with this exact problem! I've used IDT's Codon Optimization Tool quite a bit to great success. You simply need to input your desired amino acid sequence and select expression organism, and the program will give you a recommended DNA sequence.

Leanne Dooley

Thanks so much Chris. I will give IDT a try.

Kind regards

Leanne