I have some recombinant DNA that I would like to have expressed in fairly large amounts (~50mg). The protein is for a putative vaccine.
In my view Sangon Biotech is a good choice.
Thank you Libing Fu
I will contact them.
You could contact Crelux, they might be able to help you.
http://www.crelux.com/our-solutions/#prime-protein
Is it a viral or bacterial protein?
It is bacterial and therefore could be expressed by E.coli, you could try to contact: http://www.giottobiotech.com/
in generall they are quite cheap.
good luck
Manuele
Thanks Manuele
I'll check them out.
I would like to use a glycine- serine linker to link a FLAG-tag to the N-terminus of a recombinant protein. I'm not sure which codons for glycine are optimum for expression in HEK-293t cells. Any...
10 November 2019 2,605 2 View
I have a source of live pertussis cells thanks to ReseachGate. I'm now looking for a protocol to kill them using formalin.
02 March 2017 2,418 4 View
What cell densities do you recommend.
01 February 2017 4,310 4 View
I want to use the killed bacteria as an additional adjuvant in a vaccine trial in rats.
01 February 2017 5,560 2 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?
02 March 2021 9,706 5 View
I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...
02 March 2021 6,270 3 View
I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why
02 March 2021 1,388 3 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
Dear respective researchers, I was asked by my institution to be vaccinated this week using the sinovac vaccine. However, from some of the information I got, this vaccine is not recommended for...
01 March 2021 7,781 6 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
Hello every body Does any one has any idea to help me choose a reasonable basis set for intermediate metal ions (i.e Fe2+, Mn2+, Cu2+ and exc.) interactions with guanine or other DNA organic...
01 March 2021 6,187 2 View
Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...
28 February 2021 2,723 4 View