Leanne Dooley

5 Questions 9 Answers 0 Followers

Questions related from Leanne Dooley

Can someone recommend a DNA sequence for a glycine/serine linker for optimal fusion protein expression in HEK293t cells?

I would like to use a glycine- serine linker to link a FLAG-tag to the N-terminus of a recombinant protein. I'm not sure which codons for glycine are optimum for expression in HEK-293t cells. Any...

11 November 2019 2,688 2 View

Can any one recommend a company that produces proteins of good purity for a reasonable price?

I have some recombinant DNA that I would like to have expressed in fairly large amounts (~50mg). The protein is for a putative vaccine.

08 August 2017 6,698 5 View

Does anyone have a protocol for formalin-killed Bordetella pertussis cells

I have a source of live pertussis cells thanks to ReseachGate. I'm now looking for a protocol to kill them using formalin.

03 March 2017 2,472 4 View

Does anyone know where I can get heat-killed or formalin-killed Bordetella pertussis cells?

I want to use the killed bacteria as an additional adjuvant in a vaccine trial in rats.

02 February 2017 5,611 2 View

Does anyone have any experience using Click-iT EdU imaging kits for lymphocyte proliferation assays, if so what cell culture media do you recommend?

What cell densities do you recommend.

02 February 2017 4,364 4 View