I have treated many time my RNA sample which was DNA contaminated when I used DNA 4 U for 5ug RNA then it also chop down my RNA sample when i use 0.2 U for 5 ug RNA then RNA was clear two bands
Vishnu Shukla
I dont know how can DNAase cut RNA molecule, but while Performing DNAase treatment be careful of last step where you add inactivation agent and centrifuge. As little amount of inactivation agent in rescued RNA can eventually degrade it.
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Kristian Bruun Laursen
DNAse reagents frequently contain trace amounts of RNAse. Make sure you use an RNAse free DNAse reagent.
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Ashok Kumar
It is not possible if you will use RNAse free DNAase and always precipitate DNAase reagents.
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