At what density i should seed my cardiomyocytes (isolated from neonatal mice) in a 35mm culture dish?

at what density i should seed cardiomyocytes (isolated from neonatal mice) in a 35mm culture dish? and what should be the final volume (0.5, 1 or 2ml )? i have to use this for ROS detection using...

03 April 2018 5,139 2 View

Can someone give me the protocol to study mitochondrial Calcium overload, in adult cardiomyocytes?

i need to check calcium overload in adult cardiomyocytes from AMI mouse model, kindly share the protocol and relevant literature. Thanks

31 December 2017 8,767 2 View

What could be used as a positive control for reduced infarction size (myocardial ischemia)?

which drug/reagent would be the best positive control to compare and determine the effect of a plant extract (with strong antioxidant properties) on reduced infarction size (myocardial...

10 November 2017 627 4 View

SiRNA used is overexpressing the lncRNA, WHAT COULD BE THE PROBLEM?

i am trying to knockdown a lncRNA using siRNA. with lipo2000 and Opti-mem. ..This experiment has been repeated 3-4 times in lab by different people. but now when i tried to do knockdown,, lncRNA...

05 June 2017 9,894 7 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

No title

02 March 2021 3,060 3 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View