How many mice do I need to do qPCR of T cells sorted from B16 TILs?

03 February 2020 9,215 2 View

Tools for measuring patient experience in real practice?

I am interested in different tools that have been implemented in real practice for measuring patient experience (not patient satisfaction), for instance in Spain a questionnaire called IEXPAC is...

02 December 2019 6,609 8 View

I was wondering if there are qualitative articles about what leads to impulse buying?

07 October 2019 850 5 View

Should I use a compressive strain-stress test or an oscilatory frequency sweep to obtain modulus of hydrogels in DMA?

Hello, I am working with protein-based soft hydrogels, and I want to characterize the compression behaviour of my material in DMA. However, I have yet to find an adequate comparison of the best...

03 October 2019 2,559 2 View

Did I find a new species of bacteria? How can I confirm based on the ITS, rpoB, gyr and even genomic sequences?

I found a possible new species of bacteria. For the ITS DNA sequence, similarity to other bacteria of the genus ranges from 80 to 91%. Making a Neighboor-Joining tree with the ITS sequence, my...

18 August 2019 8,893 2 View

Can I use ATR FTIR on a hydrated hydrogel sample?

I want to use ATR FTIR to accompany the changes in secondary structure of a protein hydrogel by means of deconvoluting the Amide I peak. I gelled my samples and pressed a small amount between two...

17 July 2019 3,730 3 View

Does gold coated affect the EDX analysis?

I have some stubs of plant tissues that are gold-coated. I was wondering if there is a possibility to do EDS analysis. Does the energy of the K or L edge of gold affect the analysis of the lighter...

01 February 2019 3,525 6 View

Can anyone recommend good papers about interviewing psychopathic violent offenders?

I'm looking for articles in peer-reviewed journals about interviewing techniques with psychopathic offenders.

21 January 2019 2,513 2 View

Which alternative phosphorus sources I can use for S. cerevisiae cultivation rather than potassium phosphate?

Glucaric acid, my product of interest, overlaps with phosphate present in the media in the HPLC column HPX-87H (biorad). So, I was wondering if there are other known phosphorus sources that I...

08 January 2019 1,426 3 View

Is it possible to quantify protein levels from tissue material fixed with glutaraldehyde and formaldehyde?

Hi everyone, Me and a colleague of mine perfused animals with 2% glutaraldehyde, 1% formaldehyde in 0.1 M phosphate buffer. We then hemisected the brain and the right hemisphere was kept in the...

02 July 2018 1,416 3 View

What software programs can directly simulate Additive Manufacturing Directed Energy Deposition (DED)?

I understand there are a lot of software programs that can simulate DED by going through specific modeling, simulation, and commands. For me, that was time consuming and results were not accurate....

24 February 2021 4,428 3 View

I am looking for a simple software program to model 3D chemical bonds. The purpose is to generate high-quality graphical abstracts. Any suggestion?

Hello everyone: I am looking for a simple software program to model chemical bonds. The purpose is to generate a high-quality graphical abstract for a top leading journal in the field of...

23 February 2021 8,012 3 View

Which programs are to be used to register 2D image onto a 3D image data set?

I am trying to register a light micrograph onto a 3D CT data set, as I want to extract the corresponding image from the CT data set. Is there any software or program can do this accurately? I am...

21 February 2021 8,220 3 View

What is the better software for approximation of 3D surface with equations?

I have a massive of x, y, z coordinates of 2D surface in 3D space, which builded in Ansys HFSS or AutoCAD. How can I get the analitical aproximation of this surface by formulas?

21 February 2021 3,444 7 View

How can I obtain Reaction Forces in ABAQUS?

I am trying to simulate a simplified version of a beam resting on 10 points using Abaqus. The solutions I want to obtain are the Reaction Forces in each resting point or area when applying a...

21 February 2021 3,075 2 View

How to convert 2D stack images to 3D to obtain surface area of nucleus?

I have 15 slices of the nucleus at different z-heights from confocal imaging. I would like to use these slices to reconstruct the 3D nucleus and ultimately estimate the surface area of the...

20 February 2021 6,967 1 View

How to get the vector of a specific vibration mode in phonon dispersion?

For example, I have this phonon dispersion(Fig. 1), and now I would like get vector of atoms which describes TA1 in P. I know v_sim can show vibrational animation of specific mode, but I do not...

20 February 2021 4,332 4 View

Has anyone used deep learning for 2D/3D REGISTRATION ?

I am a Master student in Biomedical (Bio-electrical) Engineering and work on " 2D-3D registration ( CT and X-Ray) as my thesis. I created DRR from CT and want use CNN( Convolutional Neural...

19 February 2021 6,354 3 View

PV system design. 3d modelling and Analysis?

I have been looking for a good cost-effective tool for 3D modelling a glass-glass solar module system. I was initially considering Skelion as a tool but it does not look very promising, a bit...

13 February 2021 8,126 1 View

2D wavelet transform?

Dear all I have a 2-dimensional mode shape of one concrete beam and I put the data of mode shape in a matrix (69*3) which the first column in the matrix is x coordination of my beam and the second...

10 February 2021 3,301 3 View