How can I selectively dry etch Ti on a polymer substrate?

24 February 2021 5,378 1 View

How to identify when a gaussian program is about terminate normally?

Dear all, I am trying to optimize a bimetallic complex using # opt=tight b3lyp/lanl2dz scf=qc geom=connectivity. The program is running for around 5 days but the size of the .chk remains same...

05 February 2021 8,721 3 View

How do you create a 0.5M EDTA disodium salt solution at pH=7.4?

I have been trying to make a 0.5M EDTA solution by dissolving EDTA disodium salt dihydrate in 500mL of purified water and adjusting the pH to ~8.0 so that the salt dissolves. To aid in the...

12 October 2020 3,242 2 View

How can I increase the length of DNA fragments recovered from QIAamp DNA Micro Kit?

Hi everyone. I'm performing DNA extractions for pools of ~10 individual Daphnia magna (water fleas) using the Qiagen QIAamp DNA Micro Kit. I use a pestle to homogenise the Daphnia before entering...

07 September 2020 9,956 1 View

Where could I find3D resistivity/conductivity (ERT) opensource data?

Hi, I would like to know whether is there any open source 3D inversion resistivity/conductivity geophysical data (ERT) available. If yes, how to access it?

06 August 2020 8,209 3 View

Is it normal to get different results when you try to simulate a paper your are basing your research on?

I am simulating AODV and AOMDV in NS 2 to compare their performance in terms of Packet Delivery Ratio (PDR), Throughput, Delay and energy consumption, in order to propose a better algorithm. The...

18 July 2020 9,755 9 View

How can I assess a method that returns high-expressed genes?

Hello, I working with a method that finds high-expressed genes in a single-cell experiment. How can I assess such method? I know I can compare it with the ones in literature (t-test, regression),...

22 February 2020 3,023 2 View

How should I hydroxylate tungsten powder for organosilane grafting?

I am having trouble depositing MPTMS/APTES onto tungsten and I am concerned there are not enough hydroxyl groups on the powder surface. So far I have been exposing tungsten to water to hydroxylate...

04 February 2020 4,677 2 View

How many mice do I need to do qPCR of T cells sorted from B16 TILs?

03 February 2020 9,215 2 View

Is it possible to perform a meta-analysis for an index value?

Dear researcher community. I want to ask if it's possible to perform a meta-analysis in an index, I mean, in value without standard deviation. For instance, in the regression model, many indexes...

04 December 2019 3,797 1 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

03 March 2021 5,360 2 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View