Hi there,

As S. aureus is a Gram+ it doesn't exhibit any outer membrane. For membrane fraction preparation, cells have to be digested with lysostaphin (to degrade peptidoglycan and promote cell lysis) then sonicated (or other mechanical treatment) and then cellular fractionation is made through centrifugation. You may inspire from the following paper:

http://www.microbiologyresearch.org/docserver/fulltext/micro/150/1/45.pdf?expires=1463133334&id=id&accname=guest&checksum=3E3781305C2839C456CBD4D24915FDD4

Then usual protein extraction using detergent (DDM for instance) is performed to get rid of lipids.

Hi, here is a paper we published about how to overexpress and purify one receptor protein of S. aureus. Hopefully, you can find some useful information from it. Thanks.

"Green Fluorescent Protein (GFP)-Based Overexpression Screening and Characterization of AgrC, a Receptor Protein of Quorum Sensing in Staphylococcus aureus"

Follow any published paper. There ARE SEVERALs