Can someone check my HepG2 morphology?

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Malcolm Nobre

Hello Grace Mcintosh

The cells do not look good to me. They may not survive in culture. I have attached a link below that provide images of healthy HepG2 cells. Please do have a look.

https://www.culturecollections.org.uk/media/57228/85011430-hep-g2-images.pdf

I feel you may have to revisit your freezing protocol.

Best.

Samir Ranjan Panda

Hi Grace Mcintosh

I do agree with Malcolm Nobre. The morphology does appear different than how it should be.

If the cells have been procured from ATCC, I would suggest you to check the details in their website.

https://www.atcc.org/products/hb-8065#:~:text=Hep%20G2%20%5BHEPG2%5D%20is%20a,is%20a%20suitable%20transfection%20host.

Thanks

Samir

Grace Mcintosh

Thank you both, @Malcolm Nobre and @Samir Ranjan Panda, I have lef them to grow and will check on them tomorrow. I agree about the morphology I wasn't sure if they looked correct based on what I've seen online