Can some one provides me with plasmid that can harbor two sgRNA, with a GFP for sorting transfected cells?

Arnaud Peliace Azonpi Lemoge @Arnaud-Peliace-Azonpi-Lemoge

30 March 2016 1 3K Report

I would like to Knock out a gene in a cell line by using CRISPR/Cas9 method. In in order to increase the efficiency of the KO, I intend to use multiplex CRISPR/Cas 9 and for that, I need a plasmid that can harbor two sgRNA, with a GFP for sorting transfected cells.. In my lab , we don't have it. Can some one provides me with it please?

Hendrik de Buhr

You will find everything you need here: https://www.addgene.org/

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