Is it necessary to include an FMO for 7-AAD dye in your Flow Cytometry experiment? If not, why?

02 April 2024 0 3K Report

I'm optimizing a flow cytometer experiment and one of my three stains is a 7-AAD Live/Dead stain. The other stains in my experiment are staining for CD34+ and CD45+ cells. Is it necessary to include an FMO control for Live/Dead?

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