What effect size should i use for a meta-analysis when study data violate normality assumptions?

I'm looking for advice regarding what effect size to use for meta-analysis. Our question is whether the number of individuals in a group affects the per-capita productivity of that group. Does...

10 February 2021 5,510 8 View

What are the conflicts between EIA and environmental engineers and lawyers?

Environmental Impact Assessment (EIA) does not live up to the expectation of environmental lawyers and environmental engineers. In your own opinion, how can the EIA process therefore be made more...

03 February 2021 1,329 6 View

Multicloning in pRMC2?

Hi all, I want to express several genes in S.aureus by using the pRMC2 expression vector. I want to try to subclone some genes already cloned into pRMC2 (and their own tetO promoter) and put them...

01 February 2021 5,108 3 View

How to convert a domestic microwave assisted synthesis for dedicated instrumentation?

Hello, I just dived into the topic of microwave assisted synthesis and found a literature I would like to reproduce. The problem is in the literature, a domestic microwave oven is used which we...

27 January 2021 4,788 4 View

Can you give me an example of a faulty or egregious environmental claim that is NOT related to climate change?

I am doing lesson plans and am looking for examples of environmental claims that do not stand up to close scrutiny. There are more than enough examples of this related to climate change...so no...

18 January 2021 4,661 7 View

How do you improve retroviral expression from PG13 cells?

I have been trying to express a gene (GFP) in a retroviral system by transfecting human Phoenix-ECO cells, and transducing murine PG13 cells with the supernatant. I then sort GFP-positive PG13...

18 January 2021 476 1 View

Numerical taxonomy with SPSS?

Hi everyone, I was wondering about the possibilities of performing numerical taxonomy with SPSS software. I would be very thankful to recieve advice!! For now I have been reading about...

12 January 2021 5,549 3 View

Analytics Maturity of Logistics SMEs?

Data are important to companies. That holds for logistical companies too! With this survey we measure where logistical companies stand when it comes to "analytics". Analytics, here, is a catch-all...

13 December 2020 5,834 3 View

Do Radial Basis Function Networks trained through Gradient Descent benefit from Logistic Regression?

When training a Radial Basis Function Classifier for classification problems, we generally train the model by making use of the pseudo-inverse of the design matrix, minimizing the MSE error...

09 December 2020 2,190 1 View

How to retrieve 30 m Resolution LAI from Landsat data by combining MODIS training dataset using SVR?

I am currently working on a personal project and one of the objectives is retrieving Leaf Area Index from Landsat data using MODIS LAI product and Surface Reflectance as training data. Can someone...

30 November 2020 5,322 2 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View