We are going to do a FITX-dextran assay for assessing in vivo intestinal permeability in mice. We are also going to take out various tissue for FACS analysis, so timing is everything here.
Can we simply the store the serum (in the fridge?) and measure FITC 1 or perhaps 2 days later?
Thanks!
Hi Randi,
I probably would analyze it the same day but I also kept it in the fridge until the evening, worked just fine. Fitc-dextran should not degrade like most protein (but of course it needs to be stored away from light). Over night should theoretically be ok but I would definitely not risk 2 days (sigma aldrich says the in vivo stability is about 24h).
Hope I could help & good luck with your experiment!
Hi Sonja - it was definitely a help, thanks a lot!
I think that we will also do it in the evening on the same day, to be sure.
Thanks!
Apologies for the late question - I'm also looking into using FITC-dextran for an intestinal permeability assay. I have seen protocols that describe using serum (spun clotted blood) or plasma (blood spun with anti-coagulant), and a few protocols that require using anti-coagulant but call the samples "serum". In your experience, does it matter? I typically just collect serum samples but will process the samples with anti-coagulant if required, or if it gives cleaner results.
Sonja P Zahner as my follow up question, Can we not store the collected sample (serum/plasma) for more than 24 hours (let's say a month) before analysis. I have sample collection at different time points and I would like to run the analysis at once. Additionally, I read paper that used Serum (almost all in case of broilers a few used plasma in mice model), but I was wondering if we can also work with plasma in broilers. Any information will be helpful.
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