Sonja P Zahner

2 Questions 6 Answers 0 Followers

Questions related from Sonja P Zahner

Anti-FLAG-tag antibody for flow cytometry in mouse without major background signal issues?

Hi there, Can anybody recommend an antibody that works well to detect FLAG-tagged protein in mice with flow cytometry? I am considering to use FLAG tag for generating a transgenic mouse. HIS tag...

27 May 2016 3,500 4 View

Has anyone used one of the gene trapping constructs form KOMP to generate floxed mice and actually gotten a positive LacZ staining of targeted cells?

Me and two of my colleagues from different labs at my institute used three different constructs targeting 3 different genes. All genes seem to get deleted fairly well but unfortunately no one of...

24 March 2015 9,059 1 View