Can Raji cells produce infectious virus?

07 November 2018 1 3K Report

I've read that TPA can induce lytic genes in Raji cells but is cell free virus infectious?

Article Induction of Epstein–Barr virus (EBV) reactivation in Raji c...

It's missing a load of important ORFs and is replication deficient - Article Sequence analysis of Raji Epstein-Barr virus DNA

also

Hatfull G, Bankier A T, Barrell B G, Farrell P J. Sequence analysis of Raji Epstein-Barr virus DNA. Virology. 1988;164:334–340

It's missing BZLF2 which is needs for entry to B cells; BALF2 is truncated and is needed to get full length viral DNA production... So I don't think you could get a a productive infection in a living host. You might get it to superinfect is you used an intact EBV strain at the same time, to supply the missing gene products.

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