Will FITC labeled primary antibodies still bind their epitope (phospho-Tryosine in this case) in western blotting?
Assume an unlimited supply of primary antibody.
I feel like this would save time.
it should work is it is an anti-phospho-Tryosine, but it would be ideal to titrate the antibody to determine the best antibody concentration to use (sometimes the recommended one is not the best one)
If the target protein is not denatured, in theory, it should work. However, I advise you to refer to the package insert or website of manufacturer to check if this particulat clone/preparation had been tested in WB.
One more thing, fluorochromes can be sensitive to pH and might not work optimally outside the recommended pH range; FITC in particular, so you need to be careful. At the end of the day, you would need to validate
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