When a fungi possess both lignin degrading as well as cellulose degrading activity, then which activity will be expressed more if lignocellulosic substrate is given?
Any lignocellulosic substrate have some concentrations of lignin and more concentrations of cellulose therefore choose a substrate that have the reverse concentrations.
But Yes we all think and it was happened many times that to induce any enzyme production we have to give that substrate on which enzyme act upon. But not always it works.
Therefore you have to optimize that. I mean production optimization (in U/mg) should be determined for the answer.
In many cases, e,g. natural decompositions involve a mixture of several microorganisms - yeasts or molds or even with bacteria working together in stages. One type may degrade the lignin breaking the cellulosic structure followed by another type degrading the cellulose, etc. Very few are able to consume both lignin and cellulose simultaneously. If it happens, then the mechanism would probably be competitive between the two substrates where the fungi would choose its preferred substrate first over the other.
No, because pH is main factor and sacharification is generally carried out in acidic medium. Yes pentose and hexose can be fermented simultaneously using appropriate enzyme. If fungi is the pretremet , then I have no experience of it. From my point of sacharrifiaction is first and is to be completed ,only the fermentation is the step. You cantry , I am doubtful of toxification due to lignin which will decrease the efficiency of fermenting enzyme