Why Non-template control (NTC) gives ct value <28 in the qPCR experiments?

I am doing qPCR experiments, I am making the reaction with same water for samples, NTC, however negative control is also giving ct

01 February 2020 7,123 3 View

Is there any difference between in the efficiency of graphene oxide and reduced graphene oxide in water desalination?

Is there any difference between in the efficiency of graphene oxide and reduced graphene oxide in water desalination?

01 February 2019 4,563 4 View

If I have linoleneic acid in bottle while 25g is only written on bottle, than how can i make 50 mg/L solution from it?

I have Lenoleneic Acid. The amount written on bottle is 25g while it is a liquid. Than can calculate and make 50 mg/L solution from it.

08 September 2018 3,505 3 View

How to run multiple samples in single run inq PCR with different anealing tempretures?

I want to do qPCR of multiple genes but the problem is that the anealing temperature of multiple primer sets varies a little. How can i address this?

01 February 2018 5,424 8 View

What statistical test will be best for 16S metagenomic data for a case control study

I am analyzing my data, and I want to do statistical test for my data. I have two groups healthy and disease group (case-control study). I have carried 16S metagenomics via Illumina platform. I am...

07 August 2017 2,441 4 View

How to learn about QIIME?

I want to use QIIME for metagenomic analysis but I do not find any guideline or pipeline to follow. Do anyone have an idea about where from I can learn about QIIME, specially alpha and beta diversity.

08 September 2016 1,687 2 View

Which chemicals can be used as substitutes for Spermidine for removal of DNA during RNA isolation?

Hello all, I am working with Mango pulp to isolate Total RNA. My lab lacks Spermidine which precipitates and removes DNA from sample during first stage in RNA isolation. I am following CTAB method...

10 November 2014 4,995 5 View

Is there any molecular biology technique to distinguish the bacterial isolates of the same Genera?

I have many bacterial isolates which belong to bacillus genera. I want to know which strains of the bacillus I have

11 December 2013 7,387 12 View

How to avoid contamination from my media and plates?

I have often done plating and inoculation and all that, but right now I am facing a problem of the contamination in my media even when it is growing in unstreaked plates. I have changed the plates...

04 May 2012 5,293 10 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

No title

02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View