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Why Non-template control (NTC) gives ct value <28 in the qPCR experiments?

I am doing qPCR experiments, I am making the reaction with same water for samples, NTC, however negative control is also giving ct

01 February 2020 7,230 3 View

Is there any difference between in the efficiency of graphene oxide and reduced graphene oxide in water desalination?

01 February 2019 4,642 4 View

If I have linoleneic acid in bottle while 25g is only written on bottle, than how can i make 50 mg/L solution from it?

I have Lenoleneic Acid. The amount written on bottle is 25g while it is a liquid. Than can calculate and make 50 mg/L solution from it.

08 September 2018 3,571 3 View

How to run multiple samples in single run inq PCR with different anealing tempretures?

I want to do qPCR of multiple genes but the problem is that the anealing temperature of multiple primer sets varies a little. How can i address this?

01 February 2018 5,534 8 View

What statistical test will be best for 16S metagenomic data for a case control study

I am analyzing my data, and I want to do statistical test for my data. I have two groups healthy and disease group (case-control study). I have carried 16S metagenomics via Illumina platform. I am...

07 August 2017 2,511 4 View

Which chemicals can be used as substitutes for Spermidine for removal of DNA during RNA isolation?

Hello all, I am working with Mango pulp to isolate Total RNA. My lab lacks Spermidine which precipitates and removes DNA from sample during first stage in RNA isolation. I am following CTAB method...

10 November 2014 5,057 5 View

Can polyvinyl pyrolidone (PVP) can be used instead of polyvinyl polypyrolidone (PVPP)?

I am working on the extraction of total RNA from mango pulp. The major reason for using these compounds is to remove poly phenols from the RNA extract. so i want to know can I use polyvinyl...

09 October 2014 256 6 View

Is there any molecular biology technique to distinguish the bacterial isolates of the same Genera?

I have many bacterial isolates which belong to bacillus genera. I want to know which strains of the bacillus I have

11 December 2013 7,465 12 View

How to avoid contamination from my media and plates?

I have often done plating and inoculation and all that, but right now I am facing a problem of the contamination in my media even when it is growing in unstreaked plates. I have changed the plates...

04 May 2012 5,435 10 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View