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Why Non-template control (NTC) gives ct value <28 in the qPCR experiments?

I am doing qPCR experiments, I am making the reaction with same water for samples, NTC, however negative control is also giving ct

01 February 2020 7,123 3 View

Is there any difference between in the efficiency of graphene oxide and reduced graphene oxide in water desalination?

01 February 2019 4,563 4 View

If I have linoleneic acid in bottle while 25g is only written on bottle, than how can i make 50 mg/L solution from it?

I have Lenoleneic Acid. The amount written on bottle is 25g while it is a liquid. Than can calculate and make 50 mg/L solution from it.

08 September 2018 3,505 3 View

How to run multiple samples in single run inq PCR with different anealing tempretures?

I want to do qPCR of multiple genes but the problem is that the anealing temperature of multiple primer sets varies a little. How can i address this?

01 February 2018 5,424 8 View

What statistical test will be best for 16S metagenomic data for a case control study

I am analyzing my data, and I want to do statistical test for my data. I have two groups healthy and disease group (case-control study). I have carried 16S metagenomics via Illumina platform. I am...

07 August 2017 2,441 4 View

Which chemicals can be used as substitutes for Spermidine for removal of DNA during RNA isolation?

Hello all, I am working with Mango pulp to isolate Total RNA. My lab lacks Spermidine which precipitates and removes DNA from sample during first stage in RNA isolation. I am following CTAB method...

10 November 2014 4,995 5 View

Can polyvinyl pyrolidone (PVP) can be used instead of polyvinyl polypyrolidone (PVPP)?

I am working on the extraction of total RNA from mango pulp. The major reason for using these compounds is to remove poly phenols from the RNA extract. so i want to know can I use polyvinyl...

09 October 2014 144 6 View

Is there any molecular biology technique to distinguish the bacterial isolates of the same Genera?

I have many bacterial isolates which belong to bacillus genera. I want to know which strains of the bacillus I have

11 December 2013 7,387 12 View

How to avoid contamination from my media and plates?

I have often done plating and inoculation and all that, but right now I am facing a problem of the contamination in my media even when it is growing in unstreaked plates. I have changed the plates...

04 May 2012 5,293 10 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View