Junaid Kori

10 Questions 10 Answers 0 Followers

Questions related from Junaid Kori

Why Non-template control (NTC) gives ct value <28 in the qPCR experiments?

I am doing qPCR experiments, I am making the reaction with same water for samples, NTC, however negative control is also giving ct

02 February 2020 7,206 3 View

Is there any difference between in the efficiency of graphene oxide and reduced graphene oxide in water desalination?

02 February 2019 4,616 4 View

If I have linoleneic acid in bottle while 25g is only written on bottle, than how can i make 50 mg/L solution from it?

I have Lenoleneic Acid. The amount written on bottle is 25g while it is a liquid. Than can calculate and make 50 mg/L solution from it.

09 September 2018 3,546 3 View

How to run multiple samples in single run inq PCR with different anealing tempretures?

I want to do qPCR of multiple genes but the problem is that the anealing temperature of multiple primer sets varies a little. How can i address this?

02 February 2018 5,491 8 View

What statistical test will be best for 16S metagenomic data for a case control study

I am analyzing my data, and I want to do statistical test for my data. I have two groups healthy and disease group (case-control study). I have carried 16S metagenomics via Illumina platform. I am...

08 August 2017 2,478 4 View

How to learn about QIIME?

I want to use QIIME for metagenomic analysis but I do not find any guideline or pipeline to follow. Do anyone have an idea about where from I can learn about QIIME, specially alpha and beta diversity.

09 September 2016 1,736 2 View

Which chemicals can be used as substitutes for Spermidine for removal of DNA during RNA isolation?

Hello all, I am working with Mango pulp to isolate Total RNA. My lab lacks Spermidine which precipitates and removes DNA from sample during first stage in RNA isolation. I am following CTAB method...

11 November 2014 5,033 5 View

Can polyvinyl pyrolidone (PVP) can be used instead of polyvinyl polypyrolidone (PVPP)?

I am working on the extraction of total RNA from mango pulp. The major reason for using these compounds is to remove poly phenols from the RNA extract. so i want to know can I use polyvinyl...

10 October 2014 223 6 View

Is there any molecular biology technique to distinguish the bacterial isolates of the same Genera?

I have many bacterial isolates which belong to bacillus genera. I want to know which strains of the bacillus I have

12 December 2013 7,433 12 View

How to avoid contamination from my media and plates?

I have often done plating and inoculation and all that, but right now I am facing a problem of the contamination in my media even when it is growing in unstreaked plates. I have changed the plates...

05 May 2012 5,359 10 View