I'm unfamiliar with ESC culture. Can they be grown into confluent monolayers? Do they form epithelial adherens junctions?
I'm having trouble sorting through the literature, as pubmedding those terms comes up with a ton of noise (like ESC being differentiated into something with adherens junctions).
I've heard of small micropatterned cultures like
http://www.nature.com/nmeth/journal/v11/n8/abs/nmeth.3016.html
But I'm wanting to know if a flat uniform epithelial layer of pluripotent cells is possible. If so, is there a size limit on it?
Virtually all adherent normal cells can grow to form confluent layer. Usually after 70 % of confluence the contact inhibition could be detected. Stem cells tend to form multilayer structures at this point as well as "focal adhesion" points with multilayer structures in the very start of culture on low adhesion or not coated plates (matrix components such as laminin, collagen, fibronectin etc.).
I believed you will find small region of monolayer but will be it suitable or not depending of you aim.
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