The particle-to-protein ratio has been widely used to determine the purity of exosomes. Usually, NTA is used to measure the particle concentration (particles/mL) of the exosomes while microBCA assay is used to measure the protein concentration of the exosomes.
If I loaded exosomes with protein of interest (POI) by fusing it to an exosomal marker such as CD9, then the protein concentration of the exosomes detected by microBCA assay would be much higher resulting in a much lower particle-to-protein ratio. So is it right to use particle-to-protein ratio for measuring purity of protein-loaded exosomes?
Also, microBCA not only measures contaminant proteins in the exosome-resuspended buffer but also proteins within the exosomes including exosomal marker proteins. So I am not sure whether particle-to-protein ratio is a good way to measure the purity of the exosoms.
Please let me know what you guys think. Thanks.
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