Hi, I am a researcher working with renal cells treated with oxalate. I am facing problems when performing my western blot analysis as I found that results are not consistent. We perform western blot as a routine technique with other samples and never faced problems following BioRad western blot protocol.
We are considering that oxalate (CaOx) crystals in the sample may be interfering during the running process or in the western developing.
Does anyone has experience on this subject?